Journal: Biomedicines
Article Title: The Integrated Effects of Brivaracetam, a Selective Analog of Levetiracetam, on Ionic Currents and Neuronal Excitability
doi: 10.3390/biomedicines9040369
Figure Lengend Snippet: Stimulatory effect of BRV on the activity of the large-conductance Ca 2+ -activated K + (BK Ca ) channels in GH 3 cells. In this set of inside-out current recordings, the cells were immersed in a high-K + solution containing 1 µM Ca 2+ , where the holding potential was clamped at +60 mV. ( A ) Representative BK Ca -channel traces (blue color) obtained in the control (i.e., BRV was not present) (left side of the figure) and after the bath addition of 10 µM of BRV (right side of the figure). It should be noted that the upward deflection shows the channel opening event. The lower part (red color) of the figure shows the expanded records taken from the uppermost part of the figure; ( B ) Summary bar graph showing the open-state likelihood of the presence of BK Ca channels in the presence of BRV (10 µM), BRV (10 µM) plus TRAM39 (3 µM), BRV (10 µM) plus GAL-021 (3 µM), and BRV (10 µM) plus PF1022A (3 µM) (mean ± SEM; n = 7–8). The channel activity was measured when the membrane patch was maintained at +60 mV. * Significantly different from the control ( p < 0.05) and Ɨ significantly different from the 10 µM BRV-alone group ( p < 0.05).
Article Snippet: GAL-021 and PF1022A were acquired from MedChemExpress (Everything Biotech, New Taipei City, Taiwan); cilobradine was obtained from Cayman (Excel Biomedical, Taipei, Taiwan); 2-chloro-α, α-diphenylbenzeneacetonitrile (TRAM39) was obtained from Tocris (Union Biomed Inc., Taipei, Taiwan), and tefluthrhin, tetraethylammonium chloride and tetrodotoxin were obtained from Sigma-Aldrich (Merck Ltd., Taipei, Taiwan).
Techniques: Activity Assay, Control, Membrane
